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KMID : 0665220140270050845
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Korean Journal of Food and Nutrition
2014 Volume.27 No. 5 p.845 ~ p.850
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Inhibitory Effects of Ethanol Extracts of Some Korean Plants on Ultraviolet B-Induced DNA Damage
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Lee Seok-Hee
Ha Se-Eun
Cho Hyoung-Kwon
Park Jong-Kun
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Abstract
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In the present study, the effects of extracts from Korean plants on the DNA damage response in HaCaT cells exposed to ultraviolet B (UVB) were investigated. The activity of cells treated for 24 hr with ethanol extracts from Vaccinium spp. (VS), and Vitis vinifera L (VV) alone was similar to that of the non-treated control, but gradually decreased at concentrations above 200μg/mL. However, when post-incubation of UVB-exposed cells was carried out for 24 hr in medium containing VS or VV extracts, the cell activity increased in a concentration-dependent manner compared with that in the normal growth medium. The cell viability of UVB-exposed cells also increased when post-incubated in medium containing VS or VV extracts, in a concentration-dependent manner. Nuclear fragmentation analysis showed that post-incubation with VS or VV extracts decreased the UVB-induced apoptosis by about 10 and 13%, respectively, of that in cells post-incubated in growth medium. After 24 hr of post-incubation in medium containing VS or VV extracts, the level of CPD and 8-OHdG decreased in time- and concentration-dependent manners. Overall these results suggest that VS and VV extracts assist the survival of UVB-exposed cells, in accordance with the respective decrease in the levels of UVB-induced DNA damage.
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KEYWORD
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Vaccinium spp, Vitis vinifera L, CPD, 8-OHdG, UVB
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